Often referred to as the "body clock", circadian rhythm controls what time of day people are most alert, hungry, tired or physically primed due to a complex biological process that is not unique to humans. Circadian rhythms, which oscillate over a roughly 24–hour cycle in adaptation to the Earth's rotation, have been observed in most of the planet's plants, animals, fungi and cyanobacteria, and are responsible for regulating many aspects of organisms' physiological, behavioral and metabolic functions.

Now, scientists led by the pioneering Harvard synthetic biologist Pamela Silver, Ph.D., have harnessed the circadian mechanism found in cyanobacteria to transplant the circadian wiring into a common species of bacteria that is naturally non–circadian. The novel work, which for the first time demonstrates the transplant of a circadian rhythm, is reported in a new study in Science Advances.

Circadian Figure

"By looking at systems in nature as modular, we think like engineers to manipulate and use biological circuits in a predictable, programmable way," said Silver, who is a Core Faculty member at the Wyss Institute for Biologically Inspired Engineering at Harvard University and a Professor in the Department of Systems Biology at Harvard Medical School.

Silver's team used this methodology to successfully transplant a circadian rhythm into the bacterial species E. coli, which is widely used as a "workhorse" cell species by biologists due to how well it is understood and the ease in which E. coli can be genetically altered. The genetically engineered circadian E. coli designed by Silver could one day be used in probiotic pills as a way to monitor the gut microbiota, which is the collective and diverse set of bacterial species that flourish in the human gastrointestinal tract and contribute to many different health factors.

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