Vlad Denic

Assistant Professor of Molecular and Cellular Biology

Harvard University
NorthWest Building, Room 445.30
52 Oxford Street
Boston, MA 02115
Tel: 617-496-6381
Email: vdenic@mcb.harvard.edu

Website:

http://www.mcb.harvard.edu/denic/
Lab Size: Between 5 and 10

Summary

The Denic lab takes advantage of novel methodologies for studying large-scale genetic interactions in budding yeast as well as mass spectrometry-based characterization of natively-isolated protein complexes in order to identify the essential components required for several membrane-associated cellular processes. We then carry out targeted and systematic biochemical reconstitution strategies using the identified components in order to go from parts lists to functional and mechanistic insights.

Publications

Denic V, Dötsch V, Sinning I. (2012). ER Targeting and Insertion of Tail-Anchored Proteins by the GET Pathway. Cold Spring Harbor Review, (in press).

Denic V. (2012). A portrait of the GET pathway as a surprisingly complicated young man. Trends in Biochemical Sciences, Aug. 27 (Epub).

Wang F., Whynot A., Tung M., Denic V. (2011). The Mechanism of Tail-Anchored Protein Insertion into the ER Membrane.Molecular Cell; 43, 738-750.

Stefer S., Reitz S., Wang F., Wild K., Pang YY., Schwarz D, Bomke J, Hein C, Löhr F, Bernhard F, Denic V*., Dötsch V*., Sinning I*.Structural Basis for Tail-Anchored Membrane Protein Biogenesis by the Get3-Receptor Complex. Science; 2011 Aug. 5; 333(6043): 758-762.   *co-corresponding authors.

Denic V. (2011). No Country for Old Misfolded Glycoproteins. Molecular Cell; 42, 715-717.

Wang F., Brown E. C., Mak G., Zhuang J., Denic V. (2010). A Chaperone Cascade Sorts Proteins for Post-Translational Membrane Insertion into the ER. Molecular Cell; 40, 159-171